Coding

Part:BBa_K5325002:Design

Designed by: Hien Le, Dang Minh Anh   Group: iGEM24_MichiganState   (2024-10-01)


RPA1511_plaA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 70
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 820


Design Notes

Addition of the FLAG tag that aids protein purification and visualization on Western Blot
DNA sequence was codon optimized for expression in E. coli and Shewanella oneidensis.

Source

The part originates from the DNA sequence of Rhodopseudomonas palustris CGA009 complete sequence.
NCBI ID and link to NCBI profile: NC_005296.1

References

Hajighasemi, M., Nocek, B.P., et al. (2016). "Biochemical and Structural Insights into Enzymatic Depolymerization of Polylactic Acid and Other Polyesters by Microbial Carboxylesterases." Biomacromolecules 17(6):2027-2039