Coding
Part:BBa_K5325002:Design
Designed by: Hien Le, Dang Minh Anh Group: iGEM24_MichiganState (2024-10-01)
RPA1511_plaA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 70
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 820
Design Notes
Addition of the FLAG tag that aids protein purification and visualization on Western Blot
DNA sequence was codon optimized for expression in E. coli and Shewanella oneidensis.
Source
The part originates from the DNA sequence of Rhodopseudomonas palustris CGA009 complete sequence.
NCBI ID and link to NCBI profile: NC_005296.1
References
Hajighasemi, M., Nocek, B.P., et al. (2016). "Biochemical and Structural Insights into Enzymatic Depolymerization of Polylactic Acid and Other Polyesters by Microbial Carboxylesterases." Biomacromolecules 17(6):2027-2039